Tutorial

I. Overview:

This Databse is unique database for search and explore the potential drug targets of C. difficile.

II. How to use database

I. Input as Drug Target

Here you can select the "Database ID" or "Protein name" option for database query (refer help) and then you can type Datbase ID or Protein name in the given text box as a input and click into the "Go" button as shown in Fig. 1

Fig 1 - Input page of database for Database ID or Protein name

Result :

After you click the "Go" button on the input page, the search tool will search for the HITS that are similar to your input Keywors. The list of hits you can find in the result page (Fig 2). Select the desired one from the list. Use mouse click on "Database ID" to know the detailed informtion about the selected protein (Fig 3).

Fig 2 - Result page of database for Protein name

Fig 3 - Detail information of selected protein

Fig 4 - Sequence tab for selected protein

Fig 5 - Enzyme tab for selected protein

Fig 6 - Reaction tab for selected protein

Fig 7 - Pathway tab for selected protein

Fig 8 - Orthology tab for selected protein

Fig 9 - Visualisation tab for selected protein

Fig 10 - Download tab for selected protein
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II. Input as a Databse ID

Here you can select the "Database ID" option for database query (refer help) and then you can type Datbase ID in the given text box as a input and click into the "Go" button as shown in Fig. 11

Fig 11 - Input page of database for Database ID

Result :

After you click the "Go" button on the input page, the search tool will search for the HITS that are similar to your input Keywors. The list of hits you can find in the result page (Fig 12). Select the desired one from the list. Use mouse click on "Database ID" to know the detailed informtion about the selected protein (Fig 13).

Fig 12 - Result page of database for Database ID

After you Select the desired one from the list. You can find the detailed information in the result page. You can find more informtion on the given Tab options.

Fig 13 - Detail information of selected protein
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III. Input as a Protein name

Here you can select the "Protein name" option for database query (refer help) and then you can type Protein name in the given text box as a input and click into the "Go" button as shown in Fig. 14

Fig 14 - Input page of database for Protein name

Result :

After you click the "Go" button on the input page, the search tool will search for the HITS that are similar to your input Keywors. The list of hits you can find in the result page (Fig 15). Select the desired one from the list. Use mouse click on "Database ID" to know the detailed informtion about the selected protein (Fig 16).

Fig 15 - Result page of database for Protein name

Fig 16 - Detail information of selected protein
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Fasta format of Protein sequence :

To download the Fasta format of sequence desired protein, click on "Fasta" button of infromation page of desired protein (Fig 17).

After you click the "Fasta" button on the result page, the Fasta format of prteon will display from where you can download the Fasta format sequence of your desired protein.

Fig 17 - Fasta format of sequence of selected protein
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Proteome Table :

The proteome of all strains of C. difficile (i.e. C. difficile 630, R20291 and CD196) are given in seperate table (Fig 18). you can also retrieve desired information for protein from these tables.

Fig 18 - Proteome Tables

Here both proteome tables are connected by external links, you have to click on "protein", which will result in proteome table for the perticular proteome table of that strain (Fig 19).

Fig 19 - Proteome table

Select the desired one from the list. Use mouse click on "Database ID" to know the detailed informtion about the selected protein (Fig 20).

Fig 20 - Detail information of selected protein
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Drug Target Protein Table :

The Drug target proteins for all strains of C. difficile (i.e. C. difficile 630, R20291 and CD196) are given in seperate table (Fig 21). you can also retrieve desired information for protein from these tables.

Fig 21 - Drug target proteins Tables

Here both proteome tables are connected by external links, you have to click on "protein", which will result in proteome table for the perticular proteome table of that strain (Fig 22).

Fig 22 - Drug target proteins table

Select the desired one from the list. Use mouse click on "Database ID" to know the detailed informtion about the selected protein (Fig 23).

Fig 23 - Detail information of selected protein
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